CAS No.: | 485-47-2 |
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Formula: | C9h6o4 |
EINECS: | 207-618-1 |
Classification: | Organic Analytical Reagents |
Grade: | AR |
Specific Usage: | For Biological Purpose |
Customization: |
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Common Name | Ninhydrin | ||
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CAS Number | 485-47-2 | Molecular Weight | 178.141 |
Density | 1.7±0.1 g/cm3 | Boiling Point | 449.3±45.0 °C at 760 mmHg |
Molecular Formula | C9H6O4 | Melting Point | 250 °C (dec.)(lit.) |
MSDS | ChineseUSA | Flash Point | 239.7±25.2 °C |
Symbol | GHS07 |
Signal Word | Warning |
Density | 1.7±0.1 g/cm3 |
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Boiling Point | 449.3±45.0 °C at 760 mmHg |
Melting Point | 250 °C (dec.)(lit.) |
Molecular Formula | C9H6O4 |
Molecular Weight | 178.141 |
Flash Point | 239.7±25.2 °C |
Exact Mass | 178.026611 |
PSA | 74.60000 |
LogP | 1.07 |
Vapour Pressure | 0.0±1.2 mmHg at 25°C |
Index of Refraction | 1.737 |
Storage condition | Store at RT. |
Stability | Stable. Incompatible with amines, alkalies, strong bases. Light-sensitive. |
Water Solubility | 0.1-0.5 g/100 mL at 20 ºC |
Ninhydrin is a chemical compound with the molecular formula C9H6O4. It is an organic compound that belongs to the class of cyclic ketones known as hydrindantins. Ninhydrin is commonly used as a reagent for the detection and visualization of amino acids and proteins.
Chemical Properties: Ninhydrin appears as a yellowish crystalline solid. It is sparingly soluble in water but more soluble in organic solvents such as ethanol or aceton. Ninhydrin is sensitive to heat, light, and air and should be stored in a tightly sealed container.
Amino Acid Detection: Ninhydrin is widely used in biochemical and forensic laboratories for the identification and analysis of amino acids. It reacts with primary amines (NH2) present in amino acids to form a purple or blue-colored compound called Ruhemann's purple. This reaction is known as the ninhydrin reaction or the Ruhemann's purple test.
Protein Staining: Ninhydrin can also be used to stain proteins. When proteins are treated with ninhydrin, they undergo a similar reaction as amino acids, resulting in the formation of a purple or blue color. This staining technique is useful in protein analysis and visualization in various laboratory techniques such as paper chromatography or electrophoresis.
Forensic Applications: Ninhydrin is commonly used in forensic science for the detection of latent fingerprints. When ninhydrin is applied to a surface containing amino acids from the sweat on a fingerprint, it reacts with the amino acids, producing a visible purple or blue color. This helps in the visualization and identification of fingerprints for forensic analysis.
Stability: Ninhydrin solutions are relatively unstable and can degrade over time. They are sensitive to light and undergo a reaction known as the photodegradation of ninhydrin. To maintain the stability of ninhydrin solutions, it is common to add antioxidants or store them in amber bottles protected from light.
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